About this paper

Appears in:
Pages: 5076-5081
Publication year: 2018
ISBN: 978-84-09-05948-5
ISSN: 2340-1095
doi: 10.21125/iceri.2018.2159

Conference name: 11th annual International Conference of Education, Research and Innovation
Dates: 12-14 November, 2018
Location: Seville, Spain

ENHANCING SPECIFIC COMPETENCES IN MICROSCOPIC TECHNIQUES IN PLANT SCIENCES MASTER STUDENTS

E. García-Fortea, M. Plazas, S. Vilanova, J. Prohens, P. Gramazio

Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València (SPAIN)
Development of competences for the use of advanced research techniques is of great relevance for the formation of Master students in the broad field of Plant Sciences. In order to understand the cellular processes, students will often need to observe the morphology and internal physiology of the cells, as well as make specific stains to identify different biomolecules such as lipids, carbohydrates or proteins. For this, it is necessary to subject the plant tissues to ultrathin cuts. However, this material is commonly provided directly to the students and they never get acquainted with the previous process for getting the cut tissue slices. Our Institute offers a master's degree in Plant Breeding and Genetics, in which the basics of microscopy are imparted within the course "Instrumental techniques". In this contribution we propose the introduction to a practical lesson in the course "Instrumental techniques", consisting in the elaboration of glass blades and realization of ultrathin cuts for optical microscopy. As a starting material, we will use leaf and eggplant root tissues encapsulated in Technovit7100 resin.

The design of the practice requires 9 hours divided into three sessions, in which the students will:
a) learn how to make their own glass blades to make cuts,
b) become familiar with the use of the ultramicrotome, and
c) learn to make ultrathin cuts for the observation under the optical microscope.

This practice will allow students to understand the process prior to the use of microscopic preparations, become familiar with advanced microscope equipment and learn to work with resin blocks with encapsulated sample. This will enhance their skills for basic research on the use of microscopy techniques in Plant Sciences.
@InProceedings{GARCIAFORTEA2018ENH,
author = {Garc{\'{i}}a-Fortea, E. and Plazas, M. and Vilanova, S. and Prohens, J. and Gramazio, P.},
title = {ENHANCING SPECIFIC COMPETENCES IN MICROSCOPIC TECHNIQUES IN PLANT SCIENCES MASTER STUDENTS},
series = {11th annual International Conference of Education, Research and Innovation},
booktitle = {ICERI2018 Proceedings},
isbn = {978-84-09-05948-5},
issn = {2340-1095},
doi = {10.21125/iceri.2018.2159},
url = {http://dx.doi.org/10.21125/iceri.2018.2159},
publisher = {IATED},
location = {Seville, Spain},
month = {12-14 November, 2018},
year = {2018},
pages = {5076-5081}}
TY - CONF
AU - E. García-Fortea AU - M. Plazas AU - S. Vilanova AU - J. Prohens AU - P. Gramazio
TI - ENHANCING SPECIFIC COMPETENCES IN MICROSCOPIC TECHNIQUES IN PLANT SCIENCES MASTER STUDENTS
SN - 978-84-09-05948-5/2340-1095
DO - 10.21125/iceri.2018.2159
PY - 2018
Y1 - 12-14 November, 2018
CI - Seville, Spain
JO - 11th annual International Conference of Education, Research and Innovation
JA - ICERI2018 Proceedings
SP - 5076
EP - 5081
ER -
E. García-Fortea, M. Plazas, S. Vilanova, J. Prohens, P. Gramazio (2018) ENHANCING SPECIFIC COMPETENCES IN MICROSCOPIC TECHNIQUES IN PLANT SCIENCES MASTER STUDENTS, ICERI2018 Proceedings, pp. 5076-5081.
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