Abstract:

High performance liquid chromatography is a tremendous useful and versatile technique commonly applied in the field of the sciences. Its theoretical principles are included in the learning guide of most science degrees mainly chemistry, pharmacy, biology, biochemistry, or food science and technology among other degrees. However, this technique and its practical issues involve a great complexity which results extremely difficult to be understood by students. In a HPLC experiment, there are so many variables that it is often difficult to get a feel how each variable will affect to the separation, especially due to their interrelatedness. To show the student how the experimental parameters affect to the chromatographic separation, a large number of time-consuming experiments must be performed. This fact combined with the sheer number of students and the limited number of class hours, make it infeasible that students acquire this type of experience.

The implementation of the interactive and simulated high performance liquid chromatography computer-based in the learning methodology, allows students to explore the fundamental principles of HPLC more efficiently, as if they were in the laboratory. The HPLC simulator allows controlling and adjusting wide features of experimental parameters and displays. Chromatographic output such as type, dimensions and properties of column, solvents of mobile phases, mobile phase composition, viscosity, elution mode, gradient, temperature, volume injected, flow rate, theoretical plates… among other parameters can be simulated in the software. Consequently a new graphical chromatogram will be drawn to check the effect of the parameters modified in the chromatographic resolution.

The main advantages of the use of simulated HPLC are that they are relatively cheap, easy to use, there is no risk of instrumentation damage, no consumption of supplies, less supervision is required, and provides instant information. From the pedagogical point of view, the software of simulated HPLC not only simulates chromatography runs but also enhances mastery of instrumental technique, develop scientific reasoning abilities, increase understanding of the complexity and ambiguity of empirical work and develop practical skills.

It goes without saying that simulated HPLC does not entail the replacement of the experimental HPLC instrumentation in the laboratory. But its use, as complementary tool integrated into a thoughtful sequence of classroom instruction and laboratory experiences, gives students a comprehensive knowledge of the HPLC technique.