About this paper

Appears in:
Pages: 5486-5489
Publication year: 2014
ISBN: 978-84-617-2484-0
ISSN: 2340-1095

Conference name: 7th International Conference of Education, Research and Innovation
Dates: 17-19 November, 2014
Location: Seville, Spain

STRATEGY TO HELP UNDERGRADUATE STUDENTS TO CALCULATE ACIDITY CONSTANTS BASED ON SPECTROSCOPIC MEASUREMENTS

D. Airado Rodríguez1, F. Cañada-Cañada2, D. Gonzalez-Gomez2, J.S. Jeong2

1NOFIMA (NORWAY)
2Universidad de Extremadura (SPAIN)
In this work, a proposal for pKa calculation based on spectroscopic measurements is presented to support undergraduate students. pKa is defined as the negative logarithm of the acidic dissociation equilibrium constant (Ka), thus highly acidic compounds present low pKa values. pKa calculation of organic molecules is a key issue in many fields with important implications in real life: for instance, in pharmaceutical development, medical chemistry, or material sciences. pKa values can be calculated based on a variety of instrumental measurements. The strategy we purpose here is based on spectrophotometric and spectrofluorimetric measurements, since the target molecules possess both absorptive and fluorescent properties. The target molecules are an antibiotic of the family of fluoroquinolones (ciprofloxacin) and a bioactive compound naturally present in grapes and wine (resveratrol-3-O-glucoside). Compounds from such a different families have been selected to show students the possibilities and importance of pKa calculation in different fields. The experimental protocol implies the preparation of solutions of each compound at pH values ranging approximately between 2 and 12. Then, the acid and basic forms of each compound are spectrophotometrically and spectrofluorimetrically characterized by registering the spectra of the solutions with the lowest and the highest pH, respectively. Later, photometric and fluorimetric measurements at the typical wavelengths of the acidic and the basic forms are performed on each solution. Once the experimental part is over, two different calculation methods are proposed, namely “Stenstrom and Goldsmith” and “Wilson and Lester”.
@InProceedings{AIRADORODRIGUEZ2014STR,
author = {Airado Rodr{\'{i}}guez, D. and Ca{\~n}ada-Ca{\~n}ada, F. and Gonzalez-Gomez, D. and Jeong, J.S.},
title = {STRATEGY TO HELP UNDERGRADUATE STUDENTS TO CALCULATE ACIDITY CONSTANTS BASED ON SPECTROSCOPIC MEASUREMENTS},
series = {7th International Conference of Education, Research and Innovation},
booktitle = {ICERI2014 Proceedings},
isbn = {978-84-617-2484-0},
issn = {2340-1095},
publisher = {IATED},
location = {Seville, Spain},
month = {17-19 November, 2014},
year = {2014},
pages = {5486-5489}}
TY - CONF
AU - D. Airado Rodríguez AU - F. Cañada-Cañada AU - D. Gonzalez-Gomez AU - J.S. Jeong
TI - STRATEGY TO HELP UNDERGRADUATE STUDENTS TO CALCULATE ACIDITY CONSTANTS BASED ON SPECTROSCOPIC MEASUREMENTS
SN - 978-84-617-2484-0/2340-1095
PY - 2014
Y1 - 17-19 November, 2014
CI - Seville, Spain
JO - 7th International Conference of Education, Research and Innovation
JA - ICERI2014 Proceedings
SP - 5486
EP - 5489
ER -
D. Airado Rodríguez, F. Cañada-Cañada, D. Gonzalez-Gomez, J.S. Jeong (2014) STRATEGY TO HELP UNDERGRADUATE STUDENTS TO CALCULATE ACIDITY CONSTANTS BASED ON SPECTROSCOPIC MEASUREMENTS, ICERI2014 Proceedings, pp. 5486-5489.
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